00:34:04 *** anachoret (n=iosif@24-117-100-56.cpe.cableone.net) has joined #bioinformatics 00:43:15 *** zenkk (n=zenkk@c-76-26-86-213.hsd1.wv.comcast.net) has joined #bioinformatics 00:43:35 *** bruno has quit (Remote closed the connection) 00:54:34 *** anachoret has quit ("Ex-Chat") 00:56:46 *** zenkk has parted #bioinformatics ("Leaving") 04:06:53 *** slaydragon (n=roop@59.92.146.224) has joined #bioinformatics 04:16:26 *** Splicer has quit () 04:27:18 *** slaydragon has quit (Read error: 60 (Operation timed out)) 04:33:31 *** slaydragon (n=roop@59.92.188.26) has joined #bioinformatics 04:52:59 hi 04:53:04 No. 04:53:31 yes :) 04:53:55 yes and no in fact :) 05:14:24 *** slaydragon has quit (Remote closed the connection) 05:35:13 *** toru (n=gioby@host169-195-dynamic.18-79-r.retail.telecomitalia.it) has joined #bioinformatics 06:20:15 *** synthasee has quit (Read error: 110 (Connection timed out)) 06:32:09 hi 06:32:22 kanzure, ? 06:36:42 hi faceface 06:37:09 hi faceface 06:37:18 arf wrong tab 06:56:26 *** slaydragon (n=roop@59.92.137.70) has joined #bioinformatics 07:15:32 Hey Dan. 08:04:35 yo p 08:04:38 hey kanzure 08:14:19 *** jm (n=jm@p57B9EAAC.dip.t-dialin.net) has joined #bioinformatics 08:25:46 *** clarezoe (n=clarezoe@clarezoe.csbnet.se) has joined #bioinformatics 09:06:24 *** clarezoe has quit (Read error: 104 (Connection reset by peer)) 09:43:04 *** slaydragon has quit (Read error: 110 (Connection timed out)) 09:54:52 *** highvoltz has quit () 10:02:33 *** synthase (n=synthase@68.63.20.12) has joined #bioinformatics 10:57:25 *** highvoltz (i=rogers@bling.bling.org) has joined #bioinformatics 11:29:04 *** rogers (i=rogers@bling.bling.org) has joined #bioinformatics 11:29:12 *** synthasee (n=synthase@68.63.20.12) has joined #bioinformatics 11:29:43 *** highvoltz has quit (Remote closed the connection) 11:37:06 *** synthase has quit (No route to host) 11:40:22 *** Lynx_ (n=lynx@tina-10-4.genetik.uni-koeln.de) has joined #bioinformatics 11:40:34 hey! 11:40:39 hoi 11:41:14 would anyone know of a script / tool that i can use for in silico pcr checking? like the one ncbi has on their site. basically i just want blasted primers checked for potential pcr product generation. 11:41:21 *** slaydragon (n=roop@59.92.187.144) has joined #bioinformatics 11:41:33 and, umm, am too lazy to write that part myself ;) 11:42:18 http://www.ncbi.nlm.nih.gov/projects/primer-blast/ ? 11:42:35 <_dan> mvn install 11:42:39 <_dan> no 11:42:40 <_dan> no 11:42:48 <_dan> IRC does not respond to maven commands 11:43:01 too bad _dan 11:43:04 :) 11:43:20 pingou: yeah, exaclty like that, but i want to run it locally 11:43:34 Lynx_: primer3 ? 11:44:00 pingou: yeah, i use primer3 to find the primers, but then i want to blast them against the genome to make sure there will be only one product 11:44:34 so i really just need a script that compares the hits for the primers against each other and decides if they are close enough for a pcr to work 11:45:04 i don't think ncbi makes theirs available, at least i could not find it on the ftp site 11:45:27 hm I think you could work on the website through perl 11:45:44 would a local blast make it ? 11:46:13 yes, the blast is not my problem, it is the processing of the results 11:47:04 hmm, one can request the software for the UCSC In-Silico PCR 11:47:16 maybe i'll just do that and see how they do it 11:47:53 *** synthasee has quit (Connection timed out) 13:25:17 *** craigoz has quit ("Leaving.") 13:26:26 *** craigoz (n=craigo@202.63.56.72) has joined #bioinformatics 13:26:39 *** craigoz has quit (Client Quit) 13:40:56 *** toru has quit (Remote closed the connection) 14:23:35 *** Lynx_ has quit (" HydraIRC -> http://www.hydrairc.com <-") 14:41:20 *** slaydragon has quit ("Kotta Ko") 14:47:27 *** toru (n=gioby@host158-176-dynamic.55-82-r.retail.telecomitalia.it) has joined #bioinformatics 15:15:07 *** toru has quit ("Ex-Chat") 16:43:58 *** breinbaas has quit (Client Quit) 16:45:06 *** breinbaas (i=er@xs7.xs4all.nl) has joined #bioinformatics 17:49:00 *** pingou has quit (Read error: 110 (Connection timed out)) 17:58:38 *** rogers has quit () 18:10:50 *** highvoltz (i=rogers@bling.bling.org) has joined #bioinformatics 19:16:40 *** mercutio22 (n=htorres@c95181ec.virtua.com.br) has joined #bioinformatics 19:54:39 *** kanzure has parted #bioinformatics ("Leaving.") 21:06:43 *** synthase (n=synthase@68.63.20.12) has joined #bioinformatics 21:41:41 *** synthase has quit (Read error: 110 (Connection timed out)) 22:03:17 *** synthase (n=synthase@68.63.20.12) has joined #bioinformatics 22:47:52 *** jm has quit (Read error: 110 (Connection timed out)) 23:52:26 *** craigoz (n=craigo@202.63.56.72) has joined #bioinformatics 23:58:56 *** craigoz has quit (Remote closed the connection)